ASTM F / FM – Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using. ASTM F / FM – ASTM International (ASTM). Title. “Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by . STANDARD USED: ASTM F, Standard Test Method for Resistance of Materials Used in Protective. Clothing to Penetration by Blood-Borne Pathogens .

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The type must be asttm. Clothing for protection against contact with blood and body fluids — Determination of resistance of protective clothing materials to penetration by blood-borne pathogens – Test method using Phi-X bacteriophage.

These diseases can be caused by a wide variety of microorganisms and can pose a significant risk to health and life. The integrity of the protective barrier may also be compromised during use by such effects as flexing and abrasion 8. This method is not effective for testing protective clothing materials that are internally coated by a thick coating that can absorb the liquid containing the test virus. This standard does not purport to address all of the safety concerns, if any, associated with its use.

A retaining screen is not used to support the specimen. Inferences for protection from other pathogens must be assessed on a case-by-case basis. A definitive procedure that produces a test result: It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

The test is performed in a chamber of two compartments, separated by the material evaluated. D Test Method for Retention Characteristics of 0. Examples of test methods include, but are not limited to: Any visual or virological evidence of penetration will indicate the inability of the material to resist penetration.

A retaining screen is used to support the specimen. Many factors can effect the wetting and penetration characteristics of body fluids, such as: A precision and bias statement shall be reported at the end of a test method.

Glove directives and norms list – SHIELD Scientific : SHIELD Scientific

Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. Trace Laboratories – Denver, CO, Genetic testing – Human gene mutations diseases, neoplasias and pharmacogenetics Biocidal activities with disinfectants 59 accredited tests Cosmetics Microbiology Laboratory of control authorized by AEMPS 8 accredited tests Toxicology – Biological evaluation of medical devices; Cosmetics; Biocides; MPCA products 19 accredited tests Clostridium botulinum tests Paternity tests in any species, including humans Viruses in water and shellfish Water Microbiology.


The possible alterations of the protective material due to physical, chemical or thermal effects should be taken into account, since in this case they would negatively influence the behavior of the material, so that in case it can occur, the tests should be carried out before exposures to such material, physical, chemical or thermal conditions that may deteriorate it.

The manufacturer must indicate the possibility that the material under test can be altered by thermal treatment wet sterilizationso as not to do so in the event that it can be altered. Examples of body protection include laboratory coats, coveralls, vests, jackets, aprons, surgical gowns and full body suits.

Please see the full standard for complete details. To perform the method, samples of the materials are exposed to a nutrient broth containing the bacteriophage virus Phi-X, as a virus surrogated by its characteristics similar in size to the hepatitis C virus, and by extension to the hepatitis B virus and of human immunodeficiency virus these viruses are not used because of their difficulty to be cultured in laboratories.

Materials passing Test Method F should then be tested against bacteriophage penetration using this test method to verify performance.

Wstm method described in these standards, evaluates the effectiveness of materials used in the manufacture of protective garments against bloodborne pathogens using a surrogate virus suspended in a liquid, simulating the spontaneous contact conditions in a sanitary environment.

Health professionals who treat and care for patients can be exposed to biological fluids that can transmit diseases. Some studies, however, suggest that mechanical pressures exceeding kPa [50 psig] can occur during actual clinical use 56. The pressure used under normal conditions corresponds to D Test Method for Retention Characteristics of 0.

The surface tension range for blood and body fluids excluding saliva is approximately 0. Ecotoxicology – Test with algae, Daphnia magna and fishes Environmental microbiology Plants microbiology Marine biotoxins toxins in fish and bivalve molluscs DSP, PSP, NSP, ciguatoxins Aquaculture infectious diseases Molecular diagnosis Fertilizers Microbiology Paternity tests in any species, including humans Identification of animal species and sex in meat or fish products.

Do not use Google Chrome Contact. The material used in the protective garment will pass or fail in the test, depending on whether it resists penetration is not traversed or allows it to be traversed. Genetic Testing – Human gene mutations diseases, neoplasias and pharmacogenetics Clostridium botulinum tests Molecular veterinary microbiology Tests for movement of animals South Africa, Australia, India, New Zealand Food microbiology Viruses in water and shellfish Water Microbiology Zebra mussel Dreissena polymorphaother mussels and intermediate hosts molluscs Bulinus spp.

Work Item s – proposed revisions of this standard. The pieces of the evaluated materials are recommended to have about 75 mm on each side, of which a 57 mm diameter circle is exposed.


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To protect themselves from infectious agents transmitted by blood, health personnel should wear protective clothing made of materials that prevent microbes or viruses from passing through.

Work Item s – proposed revisions of this standard. This qstm method is used to measure the resistance of materials used in protective clothing to penetration by blood-borne pathogens using a surrogate microbe under conditions of continuous liquid contact. The test f1761 carried out observing the penetration of liquid without pressure for 5 minutes, followed by 1 minute at the indicated pressure 1.

The test includes a positive control consisting of a membrane with a pore slightly greater than the diameter of the bacteriophage 0. To help simulate f6171 wetting characteristics of blood and body fluids, the surface tension of the Phi-X Bacteriophage challenge suspension is adjusted to approximate the lower end of this surface tension range.

Link to Active This link will always route to the current Active version of the standard. This includes bullet-proof clothing, general protective clothing and full body ensembles that protect from cuts, radiation, temperature extremes, hot splashes from molten metals and other hot liquids, potential impacts from tools, machinery and materials and hazardous chemicals.

The visual detection technique of this wstm method is supplemented with a biologically based assay capable of detecting virus under the specified f16771 conditions.

Therefore, it is important to understand that this test method does not simulate all the physical stresses and pressures that might be exerted on protective clothing materials during actual use. In case a non-sterile material is used, the test will include the corresponding controls to exclude the presence of the bacteriophage in the materials evaluated.

If these conditions are of concern, the performance of protective clothing materials should be evaluated for Phi-X Bacteriophage penetration following an appropriate preconditioning technique representative of the expected conditions of use.

This method is specifically defined for a penetration model of hepatitis B virus, hepatitis C virus and human immunodeficiency virus, and it is considered by extension that if it resists penetration by these viruses, it will also ast, penetration by larger microorganisms like bacteria.

Examples of body protection include laboratory coats, coveralls, vests, jackets, f16711, surgical gowns and full body suits Medical protective clothing. Ecotoxicology – Test with algae, Daphnia magnaCrustaceans Palaemon spp.

Test Method F uses the same penetration test cell and technique, but exposes material specimens to synthetic blood with visual detection of liquid penetration.